Coding

Part:BBa_K2635006

Designed by: Hsuan Cheng, Ching-Lin Kao, Yi-Chien Chuang   Group: iGEM18_NTHU_Formosa   (2018-10-03)


tTA

The tetracycline transactivator (tTA) is the main character in Tetracycline-controlled gene expression. tTA binds to TetO operator, which regulates the expression of a transgene. We use this system to regulate the transgene of our proteins, which emit signal as output.

In our design, tTA is a combination of tTa binding site and VP16, a transcription activator which triggers conformational change upon binding to the promoter. When tTA is released, it binds to TRE3G, containing several TetO sequence and a minimal promoter, tetracycline response element TRE. The binding of tTA and TRE3G will trigger the expression of downstream gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 642
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None